Introducing the
NEBNext Direct
®
BRCA1/BRCA2 Panel
The NEBNext Direct BRCA1/BRCA2 Panel enriches
the complete exon content of BRCA1 and BRCA2
genes for next generation sequencing analysis.
NEBNext Direct employs a unique, single-day workflow
that hybridizes baits directly to genomic DNA without
the need for upfront library preparation, and drives
specificity through enzymatic removal of off-target
sequence. The BRCA1/BRCA2 panel demonstrates
extremely high specificity for target regions and
unmatched target coverage uniformity relative to
PCR-based enrichment. The NEBNext Direct BRCA1/
BRCA2 Panel allows highly sensitive calling of germline
and somatic variants across a broad range of DNA
inputs, while maximizing sequencer efficiency.
Visit NEBNextDirect.com to learn more and
to request a sample.
Target with
precision.
be INSPIRED
drive DISCOVERY
stay GENUINE
The NEBNext Direct BRCA1/BRCA2 Panel delivers
highly efficient enrichment of BRCA1 and BRCA2 coding
regions with a high percentage of reads mapping to targets
This histogram shows the % of reads aligned to the human genome, and the % of
reads mapped to the targets included in the BRCA1/BRCA2 Panel across different
input DNA amounts. 10 ng, 100 ng and 1 µg of purified genomic DNA was
enriched using the NEBNext Direct BRCA1/BRCA2 Panel. Sequencing reads
were generated on an llumina
®
MiSeq
®
with 2 x 75 bp reads, 8 bp Sample ID,
and 12 bp unique molecule ID. Sequencing read alignments were performed with
BWA- MEM and PCR duplicates were filtered using the UMIs.
0
10
20
30
40
50
60
70
80
90
100
Aligned
Percent
of
Reads
(%)
Reads mapped
to targets
10 ng
100 ng
1 µg
For research use only; not intended for diagnostic use.
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by
New England Biolabs, Inc. For more information, please email us at gbd@neb.com.
The use of these products may require you to obtain additional third party intellectual property rights for
certain applications.
ILLUMINA
®
and MISEQ
®
are registered trademarks of Illumina, Inc.
© Copyright 2017, New England Biolabs, Inc.; all rights reserved.
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at
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